High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37308687.
- Also identified by DOI 10.1038/s41587-023-01825-5 and PMC identifier 11021190.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
Medical subject headings
- Liposomes
- Nanoparticles