Low-level repressive histone marks fine-tune gene transcription in neural stem cells.

Abstract

Coordinated regulation of gene activity by transcriptional and translational mechanisms poise stem cells for a timely cell-state transition during differentiation. Although important for all stemness-to-differentiation transitions, mechanistic understanding of the fine-tuning of gene transcription is lacking due to the compensatory effect of translational control. We used intermediate neural progenitor (INP) identity commitment to define the mechanisms that fine-tune stemness gene transcription in fly neural stem cells (neuroblasts). We demonstrate that the transcription factor Fruitless^C (Fru^C) binds <i>cis</i>-regulatory elements of most genes uniquely transcribed in neuroblasts. Loss of <i>fru^C</i> function alone has no effect on INP commitment but drives INP dedifferentiation when translational control is reduced. Fru^C negatively regulates gene expression by promoting low-level enrichment of the repressive histone mark H3K27me3 in gene <i>cis</i>-regulatory regions. Identical to <i>fru^C</i> loss-of-function, reducing Polycomb Repressive Complex 2 activity increases stemness gene activity. We propose low-level H3K27me3 enrichment fine-tunes gene transcription in stem cells, a mechanism likely conserved from flies to humans.