Closed-loop fluid-fluid immiscibility in binary lipid-sterol membranes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37314933.
- Also identified by DOI 10.1073/pnas.2216002120 and PMC identifier 10288576.
- Licence recorded as CC BY-NC-ND.
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Abstract
We present two binary lipid-sterol membrane systems that exhibit fluid-fluid coexistence. Partial phase diagrams of binary mixtures of dimyristoylphosphatidylcholine with 25-hydroxyxholesterol and 27-hydroxycholesterol, determined from small-angle X-ray scattering and fluorescence microscopy studies, show closed-loop fluid-fluid immiscibility gaps, with the appearance of a single fluid phase both at higher and lower temperatures. Computer simulations suggest that this unusual phase behavior results from the ability of these oxysterol molecules to take different orientations in the membrane depending on the temperature.