Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37327339.
- Also identified by DOI 10.1126/sciadv.adf5464 and PMC identifier 10275599.
- Licence recorded as CC BY-NC.
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Abstract
In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45<sup>-</sup> nonimmune cells and 196,473 CD45<sup>+</sup> immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. <i>F3</i><sup>+</sup> fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, <i>MCAM</i><sup>+</sup> fibroblasts enriched in liver metastatic tumors might promote generation of CD8_CXCL13 cells through Notch signaling. In summary, we extensively analyzed the transcriptional differences of cell atlas between primary and liver metastatic tumors of CRC by single-cell and spatial transcriptome RNA sequencing, providing different dimensions of the development of liver metastasis in CRC.
Medical subject headings
- Colorectal Neoplasms
- Liver Neoplasms
- Colonic Neoplasms