RNA Editing as a General Trait of Ebolaviruses.
basic_science · Level V
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- Record sourced from PubMed, PMID 37348869.
- Also identified by DOI 10.1093/infdis/jiad228 and PMC identifier 10651210.
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Abstract
RNA editing has been discovered as an essential mechanism for the transcription of the glycoprotein (GP) gene of Ebola virus but not Marburg virus. We developed a rapid transcript quantification assay (RTQA) to analyze RNA transcripts generated through RNA editing and used immunoblotting with a pan-ebolavirus monoclonal antibody to confirm different GP gene-derived products. RTQA successfully quantified GP gene transcripts during infection with representative members of 5 ebolavirus species. Immunoblotting verified expression of the soluble GP and the transmembrane GP. Our results defined RNA editing as a general trait of ebolaviruses. The degree of editing, however, varies among ebolaviruses with Reston virus showing the lowest and Bundibugyo virus the highest degree of editing.
Medical subject headings
- Ebolavirus
- Hemorrhagic Fever, Ebola