Single-cell m<sup>6</sup>A mapping in vivo using picoMeRIP-seq.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37349523.
- Also identified by DOI 10.1038/s41587-023-01831-7 and PMC identifier 10739642.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Current N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m<sup>6</sup>A RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying m<sup>6</sup>A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m<sup>6</sup>A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.
Medical subject headings
- Zebrafish
- RNA