Platelet-Rich Plasma Composite Organohydrogel with Water-Locking and Anti-Freezing to Accelerate Wound Healing.

Abstract

Hydrogels have gained impressive attention in biological medicine due to their excellent biosafety, softness, and varied functional components. However, conventional hydrogels have inherent defects, such as low tensile strength, weak water-locking, and poor anti-freezing. In tissue engineering, once the hydrogel loses water or freezes, it will harden the interaction interfaces and destroy the nascent granulation tissue. Herein, based on the design concept of "hard frame-soft penetration", a composite adhesive organohydrogel is fabricated by introducing bacterial cellulose and platelet-rich plasma (PRP) into a poly-N-(tris[hydroxymethyl]methyl)acrylamide (THMA)/N-acryloyl aspartic acid (AASP) hybrid gel network infiltrated with glycerol/water binary solvent. The resultant organohydrogels exhibit excellent antifreeze properties at low temperatures (-80 °C) and demonstrate stable long-term water retention (91%) in the open environment within 12 days and can adhere firmly to the tissues by the action of "hydrogen bond clusters". Additionally, the introduction of bacterial cellulose matrix endows the organohydrogel with high tensile strength similar to that of skin. In vivo, the PRP-loaded organohydrogel can release a variety of growth factors to accelerate the wound healing process through collagen deposition and angiogenesis. Altogether, this strategy will extend the life of the hydrogel in some harsh medical environments.