Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37468504.
- Also identified by DOI 10.1038/s41467-023-40065-8 and PMC identifier 10356854.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT.
Medical subject headings
- DNA
- Single Molecule Imaging