Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37474809.
- Also identified by DOI 10.1038/s41592-023-01958-0 and PMC identifier 10406603.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels. Open-3DSIM also offers the capacity to extract dipole orientation, paving a new avenue for interpreting subcellular structures in six dimensions (xyzθλt). The platform is available as MATLAB code, a Fiji plugin and an Exe application to maximize user-friendliness.
Medical subject headings
- Microscopy
- Lighting