Greengenes2 unifies microbial data in a single reference tree.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37500913.
- Also identified by DOI 10.1038/s41587-023-01845-1 and PMC identifier 10818020.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Studies using 16S rRNA and shotgun metagenomics typically yield different results, usually attributed to PCR amplification biases. We introduce Greengenes2, a reference tree that unifies genomic and 16S rRNA databases in a consistent, integrated resource. By inserting sequences into a whole-genome phylogeny, we show that 16S rRNA and shotgun metagenomic data generated from the same samples agree in principal coordinates space, taxonomy and phenotype effect size when analyzed with the same tree.
Medical subject headings
- RNA, Ribosomal, 16S
- Phylogeny
- Metagenomics