Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37537501.
- Also identified by DOI 10.1038/s41587-023-01893-7 and PMC identifier 11180605.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5-9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins.
Medical subject headings
- Photobleaching
- Luminescent Proteins