Precision RNAi using synthetic shRNAmir target sites.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37552050.
- Also identified by DOI 10.7554/eLife.84792 and PMC identifier 10409502.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.
Medical subject headings
- RNA Interference