<i>In situ</i>biosensing technologies for an organ-on-a-chip.

Abstract

The<i>in vitro</i>simulation of organs resolves the accuracy, ethical, and cost challenges accompanying<i>in vivo</i>experiments. Organoids and organs-on-chips have been developed to model the<i>in vitro</i>, real-time biological and physiological features of organs. Numerous studies have deployed these systems to assess the<i>in vitro</i>, real-time responses of an organ to external stimuli. Particularly, organs-on-chips can be most efficiently employed in pharmaceutical drug development to predict the responses of organs before approving such drugs. Furthermore, multi-organ-on-a-chip systems facilitate the close representations of the<i>in vivo</i>environment. In this review, we discuss the biosensing technology that facilitates the<i>in situ</i>, real-time measurements of organ responses as readouts on organ-on-a-chip systems, including multi-organ models. Notably, a human-on-a-chip system integrated with automated multi-sensing will be established by further advancing the development of chips, as well as their assessment techniques.