Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37603759.
- Also identified by DOI 10.1073/pnas.2302997120 and PMC identifier 10469016.
- Licence recorded as CC BY-NC-ND.
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Abstract
Tissue macrophages, including microglia, are notoriously resistant to genetic manipulation. Here, we report the creation of Adeno-associated viruses (AAV) variants that efficiently and widely transduce microglia and tissue macrophages in vivo following intravenous delivery, with transgene expression of up to 80%. We use this technology to demonstrate manipulation of microglia gene expression and microglial ablation, thereby providing invaluable research tools for the study of these important cells.
Medical subject headings
- Dependovirus
- Microglia