Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37607185.
- Also identified by DOI 10.1371/journal.pone.0289556 and PMC identifier 10443835.
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Abstract
We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.
Medical subject headings
- Hybridization, Genetic
- MicroRNAs