DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37653118.
- Also identified by DOI 10.1038/s41592-023-01976-y and PMC identifier 10482679.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by exchange reaction, immuno-SABER. We applied SABER-IMC to image the tumor immune microenvironment in human melanoma by simultaneous imaging of 18 markers with immuno-SABER and 20 markers without amplification. SABER-IMC enabled the identification of immune cell phenotypic markers, such as T cell co-receptors and their ligands, that are not detectable with IMC.
Medical subject headings
- Diagnostic Imaging
- Melanoma