Transient Dynamic Operation of G-Quadruplex-Gated Glucose Oxidase-Loaded ZIF-90 Metal-Organic Framework Nanoparticle Bioreactors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37669541.
- Also identified by DOI 10.1021/acs.nanolett.3c02542 and PMC identifier 10540265.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Glucose oxidase-loaded ZIF-90 metal-organic framework nanoparticles conjugated to hemin-G-quadruplexes act as functional bioreactor hybrids operating transient dissipative biocatalytic cascaded transformations consisting of the glucose-driven H<sub>2</sub>O<sub>2</sub>-mediated oxidation of Amplex-Red to resorufin or the glucose-driven generation of chemiluminescence by the H<sub>2</sub>O<sub>2</sub>-mediated oxidation of luminol. One system involves the fueled activation of a reaction module leading to the temporal formation and depletion of the bioreactor conjugate operating the nickase-guided transient biocatalytic cascades. The second system demonstrates the fueled activation of a reaction module yielding a bioreactor conjugate operating the exonuclease III-dictated transient operation of the two biocatalytic cascades. The temporal operations of the bioreactor circuits are accompanied by kinetic models and computational simulations enabling us to predict the dynamic behavior of the systems subjected to different auxiliary conditions.
Medical subject headings
- DNA, Catalytic
- Metal-Organic Frameworks
- G-Quadruplexes
- Nanoparticles
- Biosensing Techniques