A computer vision based optical method for measuring fluid level in cell culture plates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37682933.
- Also identified by DOI 10.1371/journal.pone.0290951 and PMC identifier 10490923.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid's index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.
Medical subject headings
- Cell Culture Techniques
- Refraction, Ocular