CRISPR-based engineering of RNA viruses.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37703376.
- Also identified by DOI 10.1126/sciadv.adj8277 and PMC identifier 10499312.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.
Medical subject headings
- Engineering
- RNA Viruses