Targeted genome editing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37709915.
- Also identified by DOI 10.1038/s41587-023-01947-w and PMC identifier 12054351.
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Abstract
Reverse transcriptases, used in prime editing systems, exhibit lower fidelity, processivity and dNTP affinity than many DNA-dependent DNA polymerases. We report that a DNA-dependent DNA polymerase (phi29), untethered from Cas9, enables editing from a synthetic, end-stabilized DNA-containing template at up to 60% efficiency in human cells. Compared to prime editing, DNA polymerase editing avoids autoinhibitory intramolecular base pairing of the template, facilitates template synthesis and supports larger insertions (>100 nucleotides).
Medical subject headings
- Gene Editing
- DNA-Directed DNA Polymerase
- DNA