S<sup>3</sup>-CIMA: Supervised spatial single-cell image analysis for identifying disease-associated cell-type compositions in tissue.

Babaei, Sepideh; Christ, Jonathan; Sehra, Vivek; Makky, Ahmad; Zidane, Mohammed; Wistuba-Hamprecht, Kilian; Schürch, Christian; Claassen, Manfred · Patterns (N Y) · 2023

basic_science · Level V

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Abstract

The spatial organization of various cell types within the tissue microenvironment is a key element for the formation of physiological and pathological processes, including cancer and autoimmune diseases. Here, we present S<sup>3</sup>-CIMA, a weakly supervised convolutional neural network model that enables the detection of disease-specific microenvironment compositions from high-dimensional proteomic imaging data. We demonstrate the utility of this approach by determining cancer outcome- and cellular-signaling-specific spatial cell-state compositions in highly multiplexed fluorescence microscopy data of the tumor microenvironment in colorectal cancer. Moreover, we use S<sup>3</sup>-CIMA to identify disease-onset-specific changes of the pancreatic tissue microenvironment in type 1 diabetes using imaging mass-cytometry data. We evaluated S<sup>3</sup>-CIMA as a powerful tool to discover novel disease-associated spatial cellular interactions from currently available and future spatial biology datasets.