Correlative montage parallel array cryo-tomography for in situ structural cell biology.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37723245.
- Also identified by DOI 10.1038/s41592-023-01999-5 and PMC identifier 10555823.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells. To further extend the flexibility of MPACT, tilt series may be processed in their entirety or as individual tiles suitable for sub-tomogram averaging, enabling efficient data processing and analysis.
Medical subject headings
- Electron Microscope Tomography