Understanding of trabecular-cortical transition zone: Numerical and experimental assessment of multi-morphology scaffolds.

Abstract

Applications of additive manufacturing (AM) in tissue engineering develop rapidly. AM offers layer-by-layer creation of complex objects, developed to restore functionality of, or replace, damaged tissues. Porous 3D-printed functional gradient structures are of particular interest: their special architecture makes it possible to simulate the heterogeneity of the replaced tissue and, by continuously changing the mechanical properties, to avoid the concentration of stresses that can be caused by abrupt geometric changes. Such structures also allow combinations of different types of unit cells and a smooth transition between them, making design of personalised scaffolds with optimal parameters for the replacement of damaged host tissue at the interface between tissues possible. This paper presents the results of development of scaffold structures with gradients of porosity and multi-morphology using unit cells based on triply periodic minimal surfaces (TPMS). The mechanical behaviour of additively manufactured scaffold prototypes made of polylactide acid (PLA) was studied under compressive loading. Strain fields on their surface were captured using the Vic-3d Micro-DIC digital image correlation system and compared with those obtained with detailed numerical simulations, employing elastic-plastic properties of PLA, obtained in experiments. The effect of gradient parameters and unit-cell morphology on the stress distribution in scaffolds was analysed. A smooth gradient transition between cells with different morphologies was found to reduce the probability of structural failure under intense compressive loading. A good agreement between numerical results and experimental data was achieved, which justifies application of the developed approach to design of personalised bone scaffolds.