Radiolytically reworked Archean organic matter in a habitable deep ancient high-temperature brine.

Nisson, Devan M; Walters, Clifford C; Chacón-Patiño, Martha L; Weisbrod, Chad R; Kieft, Thomas L; Sherwood Lollar, Barbara; Warr, Oliver; Castillo, Julio et al. · Nat Commun · 2023

basic_science · Level V

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Abstract

Investigations of abiotic and biotic contributions to dissolved organic carbon (DOC) are required to constrain microbial habitability in continental subsurface fluids. Here we investigate a large (101-283 mg C/L) DOC pool in an ancient (>1Ga), high temperature (45-55 °C), low biomass (10<sup>2</sup>-10<sup>4</sup> cells/mL), and deep (3.2 km) brine from an uranium-enriched South African gold mine. Excitation-emission matrices (EEMs), negative electrospray ionization (-ESI) 21 tesla Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and amino acid analyses suggest the brine DOC is primarily radiolytically oxidized kerogen-rich shales or reefs, methane and ethane, with trace amounts of C<sub>3</sub>-C<sub>6</sub> hydrocarbons and organic sulfides. δ<sup>2</sup>H and δ<sup>13</sup>C of C<sub>1</sub>-C<sub>3</sub> hydrocarbons are consistent with abiotic origins. These findings suggest water-rock processes control redox and C cycling, helping support a meagre, slow biosphere over geologic time. A radiolytic-driven, habitable brine may signal similar settings are good targets in the search for life beyond Earth.