A Multifunctional Hydrogel for Simultaneous Visible H₂ O₂ Monitoring And Accelerating Diabetic Wound Healing.

Abstract

Diabetic wound is one of the chronic wounds that is difficult to heal, and effective treatment of it still confronts a great challenge. Monitoring the variation of diabetic wound microenvironment (such as hydrogen peroxide (H₂ O₂ )) can understand the wound state and guide the wound management. Herein, a multifunctional hydrogel with the abilities of monitoring the H₂ O₂ concentration, alleviating oxidative stress and promoting wound healing is developed, which is prepared by encapsulating manganese-containing bioactive glass (MnBG) and CePO₄ :Tb in biocompatible gelatin methacryloyl (GelMA) hydrogel (CPT-MnBG-Gel). On the one hand, the H₂ O₂ -dependent fluorescence quenching effect of the CePO₄ :Tb contributes to visible monitoring of the H₂ O₂ concentration of wounds via smartphone imaging, and the CPT-MnBG-Gel hydrogel can effectively monitor the H₂ O₂ level of 10.35-200 µmol L^-1 . On the other hand, MnBG can alleviate oxidative stress and promote the proliferation, migration and differentiation of fibroblasts and endothelial cells in vitro owing to the bioactive Mn and Si ions, and in vivo evaluation also demonstrates that the CPT-MnBG-Gel hydrogels can effectively accelerate wound healing. Hence, such multifunctional hydrogel is promising for diabetic wound management and accelerating wound healing.