RNA N6-methyladenosine modification-based biomarkers for absorbed ionizing radiation dose estimation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 37903783.
- Also identified by DOI 10.1038/s41467-023-42665-w and PMC identifier 10616291.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Radiation triage and biological dosimetry are critical for the medical management of massive potentially exposed individuals following radiological accidents. Here, we performed a genome-wide screening of radiation-responding mRNAs, whose N6-methyladenosine (m<sup>6</sup>A) levels showed significant alteration after acute irradiation. The m<sup>6</sup>A levels of three genes, Ncoa4, Ate1 and Fgf22, in peripheral blood mononuclear cells (PBMCs) of mice showed excellent dose-response relationships and could serve as biomarkers of radiation exposure. Especially, the RNA m<sup>6</sup>A of Ncoa4 maintained a high level as long as 28 days after irradiation. We demonstrated its responsive specificity to radiation, conservation across the mice, monkeys and humans, and the dose-response relationship in PBMCs from cancer patients receiving radiation therapy. Finally, NOCA4 m<sup>6</sup>A-based biodosimetric models were constructed for estimating absorbed radiation doses in mice or humans. Collectively, this study demonstrated the potential feasibility of RNA m<sup>6</sup>A in radiation accidents management and clinical applications.
Medical subject headings
- Leukocytes, Mononuclear
- RNA