Extracellular Vesicles From Mycoplasma gallisepticum: Modulators of Macrophage Activation and Virulence.

Abstract

Extracellular vesicles (EVs) mediate intercellular communication by transporting proteins. To investigate the pathogenesis of Mycoplasma gallisepticum, a major threat to the poultry industry, we isolated and characterized M. gallisepticum-produced EVs. Our study highlights the significant impact of M. gallisepticum-derived EVs on immune function and macrophage apoptosis, setting them apart from other M. gallisepticum metabolites. These EVs dose-dependently enhance M. gallisepticum adhesion and proliferation, simultaneously modulating Toll-like receptor 2 and interferon γ pathways and thereby inhibiting macrophage activation. A comprehensive protein analysis revealed 117 proteins in M. gallisepticum-derived EVs, including established virulence factors, such as GapA, CrmA, VlhA, and CrmB. Crucially, these EV-associated proteins significantly contribute to M. gallisepticum infection. Our findings advance our comprehension of M. gallisepticum pathogenesis, offering insights for preventive strategies and emphasizing the pivotal role of M. gallisepticum-derived EVs and their associated proteins. This research sheds light on the composition and crucial role of M. gallisepticum-derived EVs in M. gallisepticum pathogenesis, aiding our fight against M. gallisepticum infections.