Adaptation of CUT&RUN for use in African trypanosomes.
basic_science · Level V
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- Record sourced from PubMed, PMID 37988382.
- Also identified by DOI 10.1371/journal.pone.0292784 and PMC identifier 10662711.
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Abstract
This Cleavage Under Targets and Release Using Nuclease (CUT&RUN) protocol produces genomic occupancy data for a protein of interest in the protozoan parasite Trypanosoma brucei. The data produced is analyzed in a similar way as that produced by ChIP-seq. While we describe the protocol for parasites carrying an epitope tag for the protein of interest, antibodies against the native protein could be used for the same purpose.
Medical subject headings
- Trypanosoma
- Trypanosoma brucei brucei
- Trypanosomiasis, African