How we do it: the Zurich Microsurgery Lab technique for placenta preparation.

Höbner, Lara Maria; Staartjes, Victor E; Colombo, Elisa; Sebök, Martina; Regli, Luca; Esposito, Giuseppe · Acta Neurochir (Wien) · 2023

basic_science · Level V

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Abstract

Perfused placentas provide an excellent and accessible model for microvascular dissection, microsuturing and microanastomosis training - particularly in the early microsurgical learning curve. This way, a significant amount of live animals can be spared. We present the Zurich Microsurgery Lab protocol, detailing steps for obtaining, selecting, cleaning, flushing, cannulating, and preserving human placentas - as well as microsurgical training examples - in a tried-and-true, safe, cost-effective, and high-yield fashion. Our technique enables highly realistic microsurgical training (microdissection, microvascular repair, microanastomosis) based on readily available materials. Proper handling, preparation, and preservation of the perfused placenta models is key.

Medical subject headings