A monoacylglycerol lipase inhibitor showing therapeutic efficacy in mice without central side effects or dependence.

Jiang, Ming; Huizenga, Mirjam C W; Wirt, Jonah L; Paloczi, Janos; Amedi, Avand; van den Berg, Richard J B H N; Benz, Joerg; Collin, Ludovic et al. · Nat Commun · 2023

basic_science · Level V

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Abstract

Monoacylglycerol lipase (MAGL) regulates endocannabinoid 2-arachidonoylglycerol (2-AG) and eicosanoid signalling. MAGL inhibition provides therapeutic opportunities but clinical potential is limited by central nervous system (CNS)-mediated side effects. Here, we report the discovery of LEI-515, a peripherally restricted, reversible MAGL inhibitor, using high throughput screening and a medicinal chemistry programme. LEI-515 increased 2-AG levels in peripheral organs, but not mouse brain. LEI-515 attenuated liver necrosis, oxidative stress and inflammation in a CCl<sub>4</sub>-induced acute liver injury model. LEI-515 suppressed chemotherapy-induced neuropathic nociception in mice without inducing cardinal signs of CB<sub>1</sub> activation. Antinociceptive efficacy of LEI-515 was blocked by CB<sub>2</sub>, but not CB<sub>1</sub>, antagonists. The CB<sub>1</sub> antagonist rimonabant precipitated signs of physical dependence in mice treated chronically with a global MAGL inhibitor (JZL184), and an orthosteric cannabinoid agonist (WIN55,212-2), but not with LEI-515. Our data support targeting peripheral MAGL as a promising therapeutic strategy for developing safe and effective anti-inflammatory and analgesic agents.

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