Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome <i>c</i> oxidase.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38064560.
- Also identified by DOI 10.1126/sciadv.adh4179 and PMC identifier 10708180.
- Licence recorded as CC BY-NC.
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Abstract
Cytochrome <i>c</i> oxidase (C<i>c</i>O) is part of the respiratory chain and contributes to the electrochemical membrane gradient in mitochondria as well as in many bacteria, as it uses the energy released in the reduction of oxygen to pump protons across an energy-transducing biological membrane. Here, we use time-resolved serial femtosecond crystallography to study the structural response of the active site upon flash photolysis of carbon monoxide (CO) from the reduced heme <i>a<sub>3</sub></i> of <i>ba<sub>3</sub></i>-type C<i>c</i>O. In contrast with the <i>aa<sub>3</sub></i>-type enzyme, our data show how CO is stabilized on Cu<sub>B</sub> through interactions with a transiently ordered water molecule. These results offer a structural explanation for the extended lifetime of the Cu<sub>B</sub>-CO complex in <i>ba<sub>3</sub></i>-type C<i>c</i>O and, by extension, the extremely high oxygen affinity of the enzyme.
Medical subject headings
- Electron Transport Complex IV
- Carbon Monoxide