Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38066665.
- Also identified by DOI 10.1093/stmcls/sxad091 and PMC identifier 10938546.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Adult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.
Medical subject headings
- Muscle, Skeletal
- Adult Stem Cells