A monomeric StayGold fluorescent protein.

Ivorra-Molla, Esther; Akhuli, Dipayan; McAndrew, Martin B L; Scott, William; Kumar, Lokesh; Palani, Saravanan; Mishima, Masanori; Crow, Allister et al. · Nat Biotechnol · 2024

basic_science · Level V

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Abstract

StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.

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