A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38123564.
- Also identified by DOI 10.1038/s41467-023-44291-y and PMC identifier 10733313.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Bacteria of the genus Actinoplanes form sporangia that contain dormant sporangiospores which, upon contact with water, release motile spores (zoospores) through a process called sporangium dehiscence. Here, we set out to study the molecular mechanisms behind sporangium dehiscence in Actinoplanes missouriensis and discover a sigma/anti-sigma system with unique features. Protein σ<sup>SsdA</sup> contains a functional sigma factor domain and an anti-sigma factor antagonist domain, while protein SipA contains an anti-sigma factor domain and an anti-sigma factor antagonist domain. Remarkably, the two proteins interact with each other via the anti-sigma factor antagonist domain of σ<sup>SsdA</sup> and the anti-sigma factor domain of SipA. Although it remains unclear whether the SipA/σ<sup>SsdA</sup> system plays direct roles in sporangium dehiscence, the system seems to modulate oxidative stress responses in zoospores. In addition, we identify a two-component regulatory system (RsdK-RsdR) that represses initiation of sporangium dehiscence.
Medical subject headings
- Actinobacteria
- Actinoplanes