Mineral Phase Reconfiguration Enables the High Enzyme-like Activity of Vermiculite for Antibacterial Application.

Abstract

Phyllosilicates-based nanomaterials, particularly iron-rich vermiculite (VMT), have wide applications in biomedicine. However, the lack of effective methods to activate the functional layer covered by the external inert layer limits their future applications. Herein, we report a mineral phase reconfiguration strategy to prepare novel nanozymes by a molten salt method. The peroxidase-like activity of the VMT reconfiguration nanozyme is 10 times that of VMT, due to the electronic structure change of iron in VMT. Density-functional theory calculations confirmed that the upward shifted <i>d</i>-band center of the VMT reconfiguration nanozyme promoted the adsorption of H₂O₂ on the active iron sites and significantly elongated the O-O bond lengths. The reconfiguration nanozyme exhibited nearly 100% antibacterial activity toward <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>), much higher than that of VMT (<i>E. coli</i> 10%, <i>S. aureus</i> 21%). This work provides new insights for the rational design of efficient bioactive phyllosilicates-based nanozyme.