High-quality metagenome assembly from long accurate reads with metaMDBG.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38168989.
- Also identified by DOI 10.1038/s41587-023-01983-6 and PMC identifier 11392814.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We introduce metaMDBG, a metagenomics assembler for PacBio HiFi reads. MetaMDBG combines a de Bruijn graph assembly in a minimizer space with an iterative assembly over sequences of minimizers to address variations in genome coverage depth and an abundance-based filtering strategy to simplify strain complexity. For complex communities, we obtained up to twice as many high-quality circularized prokaryotic metagenome-assembled genomes as existing methods and had better recovery of viruses and plasmids.
Medical subject headings
- Metagenome
- Metagenomics