LHFPL5 is a key element in force transmission from the tip link to the hair cell mechanotransducer channel.

Abstract

During auditory transduction, sound-evoked vibrations of the hair cell stereociliary bundles open mechanotransducer (MET) ion channels via tip links extending from one stereocilium to its neighbor. How tension in the tip link is delivered to the channel is not fully understood. The MET channel comprises a pore-forming subunit, transmembrane channel-like protein (TMC1 or TMC2), aided by several accessory proteins, including LHFPL5 (lipoma HMGIC fusion partner-like 5). We investigated the role of LHFPL5 in transduction by comparing MET channel activation in outer hair cells of <i>Lhfpl5-/-</i> knockout mice with those in <i>Lhfpl5+/-</i> heterozygotes. The 10 to 90 percent working range of transduction in <i>Tmc1+/+; Lhfpl5+/-</i> was 52 nm, from which the single-channel gating force, Z, was evaluated as 0.34 pN. However, in <i>Tmc1+/+; Lhfpl5-/-</i> mice, the working range increased to 123 nm and Z more than halved to 0.13 pN, indicating reduced sensitivity. Tip link tension is thought to activate the channel via a gating spring, whose stiffness is inferred from the stiffness change on tip link destruction. The gating stiffness was ~40 percent of the total bundle stiffness in wild type but was virtually abolished in <i>Lhfpl5-/-,</i> implicating LHFPL5 as a principal component of the gating spring. The mutation <i>Tmc1</i> p.D569N reduced the LHFPL5 immunolabeling in the stereocilia and like <i>Lhfpl5-/-</i> doubled the MET working range, but other deafness mutations had no effect on the dynamic range. We conclude that tip-link tension is transmitted to the channel primarily via LHFPL5; residual activation without LHFPL5 may occur by direct interaction between PCDH15 and TMC1.