Square beams for optimal tiling in transmission electron microscopy.
Where this comes from
- Record sourced from PubMed, PMID 38238558.
- Also identified by DOI 10.1038/s41592-023-02161-x and PMC identifier 11009100.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem for dose-sensitive samples. Here, we introduce a square electron beam that can easily be retrofitted in existing microscopes, and demonstrate its application, showing that it can tile nearly perfectly and deliver cryo-electron microscopy imaging with a resolution comparable to conventional set-ups.
Medical subject headings
- Cryoelectron Microscopy