Bacterial microbiome associated with cigarette beetle Lasioderma serricorne (F.) and its microbial plasticity in relation to diet sources.

Abstract

Insect-microbial symbiosis contributes positively to the physiology of the insect and diet is considered as one important factor determining microbial symbiosis. In this study, we have characterized the microbiota of cigarette beetle, Lasioderma serricorne (Fabricius) on different diets and phases. The beetles were reared on different diet sources (exposed phase) for six generations and were reverted to their natal source (reverted phase) and further maintained for six more generations. The bacterial diversity and richness were higher in the exposed phase and once reverted to the natal source, the microbial abundance has re-assembled according to the natal diet source. There was re-assemblage of microbial composition in accordance to the diet and the bacterial cells are able to establish and proliferate on reverting to their natal source. The bacterial composition of the beetle was mainly dynamic and not transient where the bacterial cells were maintained at low abundance and were re-established according to the diet source. Overall, we found that the microbiota of cigarette beetle to be dynamic and bacterial composition to re-assemble in a diet-specific manner. The study provides insights on diet associated microbial plasticity of cigarette beetle and a further comprehensive understanding on mechanisms involved in microbial plasticity will help develop novel pest management strategies for this invasive insect pest.