An improved pathway for autonomous bioluminescence imaging in eukaryotes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38253843.
- Also identified by DOI 10.1038/s41592-023-02152-y and PMC identifier 10927554.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.
Medical subject headings
- Eukaryota
- Luminescence