Efficient prime editing in two-cell mouse embryos using PEmbryo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38321114.
- Also identified by DOI 10.1038/s41587-023-02106-x and PMC identifier 11631759.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice.
Medical subject headings
- Gene Editing
- Embryo, Mammalian