Square condenser apertures for square cameras in low-dose transmission electron microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38459386.
- Also identified by DOI 10.1038/s41592-024-02206-9.
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Abstract
In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam-sensitive samples. We present condenser aperture plates with square and rectangular shapes that improve the efficiency of area usage by 70% and enhance montage imaging for beam-sensitive specimens. We demonstrate the compatibility of these condenser aperture plates with high-resolution cryogenic TEM by reconstructing a 1.8-Å map of equine apo-ferritin.
Medical subject headings
- Microscopy, Electron, Transmission