MINSTED tracking of single biomolecules.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38480903.
- Also identified by DOI 10.1038/s41592-024-02209-6 and PMC identifier 11009101.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Here we show that MINSTED localization, a method whereby the position of a fluorophore is identified with precisely controlled beams of a STED microscope, tracks fluorophores and hence labeled biomolecules with nanometer/millisecond spatiotemporal precision. By updating the position for each detected photon, MINSTED recognizes fluorophore steps of 16 nm within <250 μs using about 13 photons. The power of MINSTED tracking is demonstrated by resolving the stepping of the motor protein kinesin-1 walking on microtubules and switching protofilaments.
Medical subject headings
- Microtubules
- Kinesins