High Frequency of Prior Severe Acute Respiratory Syndrome Coronavirus 2 Infection by Sensitive Nucleocapsid Assays.

Nkolola, Joseph P; Liu, Jinyan; Collier, Ai-Ris Y; Jacob-Dolan, Catherine; Senussi, Yasmeen; Borberg, Ella; Swank, Zoe; Walt, David R et al. · J Infect Dis · 2024

cross_sectional · Level IV

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Abstract

Prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is typically measured by nucleocapsid serology assays. In this study, we show that the Simoa serology assay and T-cell intracellular cytokine staining assay are more sensitive than the clinical Elecsys assay for detection of nucleocapsid-specific immune responses. These data suggest that the prevalence of prior SARS-CoV-2 infection in the population may be higher than currently appreciated.

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