Selective-plane-activation structured illumination microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38580844.
- Also identified by DOI 10.1038/s41592-024-02236-3.
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Abstract
The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.
Medical subject headings
- Microscopy, Fluorescence