Preparing and evaluating the anti-microbial effect of <i>Allium jesdianum</i> mouthwash on some of the most common oral microorganisms.

Abstract

Due to the increasing resistance of bacteria to antibiotics and anti-bacterial compounds in plants, <i>Allium jesdianum</i> Boiss plant extract can be used in mouthwash compounds with its anti-microbial activity. The anti-bacterial and anti-fungal activity of <i>A. jesdianum</i> mouthwash was investigated on <i>Streptococcus mutans</i>, <i>Streptococcus sanguis</i>, <i>S. salivarius</i> and <i>Candida albicans</i>, and <i>Candida tropicalis</i>. To analyse the anti-microbial effect of this mouthwash, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the broth microdilution method. The average MIC and MBC of <i>A. jesdianum</i> mouthwash for <i>S. mutans</i> were 1.56 and 3.12 (mg/ml), respectively, for <i>S. salivarius</i>, 0.25 and 0.65 (mg/ml), and for <i>S. sanguis</i>, respectively, 0.25 and 0.65 (mg/ml). The highest MIC and MBC values were for <i>S. mutans</i>, and the MIC and MBC values were equal for <i>S. sanguis</i> and <i>S. salivarius</i>. Average MIC and MBC were determined as 2.41 and 4.16 (mg/ml) for <i>C. albicans</i> and 2.34 and 5.72 (mg/ml) for <i>C. tropicalis</i>, respectively. MIC values of mouthwash were higher for <i>C. albicans</i> and MBC values for <i>C. tropicalis</i>. Our results showed a promising anti-fungal-anti-bacterial effect of <i>A. jesdianum</i> extract. <i>A. jesdianum</i> extract may be used as an alternative to chemical mouthwashes.