Customized Lanthanide Nanobiohybrids for Noninvasive Precise Phototheranostics of Pulmonary Biofilm Infection.

Li, Zhuo; Lu, Shan; Liu, Wenzhen; Chen, Zhuo; Huang, Yunmei; Li, Xingjun; Gong, Jiacheng; Chen, Xueyuan · ACS Nano · 2024

basic_science · Level V

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Abstract

A noninvasive strategy for <i>in situ</i> diagnosis and precise treatment of bacterial biofilm infections is highly anticipated but still a great challenge. Currently, no <i>in vivo</i> biofilm-targeted theranostic agent is available. Herein, we fabricated intelligent theranostic alginate lyase (Aly)-NaNdF<sub>4</sub> nanohybrids with a 220 nm sunflower-like structure (NaNdF<sub>4</sub>@DMS-Aly) through an enrichment-encapsulating strategy, which exhibited excellent photothermal conversion efficiency and the second near-infrared (NIR-II) luminescence. Benefiting from the site-specific targeting and biofilm-responsive Aly release from NaNdF<sub>4</sub>@DMS-Aly, we not only enabled noninvasive diagnosis but also realized Aly-photothermal synergistic therapy and real-time evaluation of therapeutic effect in mice models with <i>Pseudomonas aeruginosa</i> biofilm-induced pulmonary infection. Furthermore, such nanobiohybrids with a sheddable siliceous shell are capable of delaying the NaNdF<sub>4</sub> dissolution and biodegradation upon accomplishing the therapy, which is highly beneficial for the biosafety of theranostic agents.

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