Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 38662916.
- Also identified by DOI 10.1126/science.adk5518 and PMC identifier 11175973.
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Abstract
Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. We show that site-specific RNA breaks generated with type-III CRISPR complexes are repaired in human cells and that this repair can be used for programmable deletions in human transcripts to restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- RNA
- RNA, Guide, CRISPR-Cas Systems
- CRISPR-Associated Proteins