UDP-glycosyltransferases act as key determinants of host plant range in generalist and specialist <i>Spodoptera</i> species.

Abstract

Phytophagous insects have evolved sophisticated detoxification systems to overcome the antiherbivore chemical defenses produced by many plants. However, how these biotransformation systems differ in generalist and specialist insect species and their role in determining insect host plant range remains an open question. Here, we show that UDP-glucosyltransferases (UGTs) play a key role in determining the host range of insect species within the <i>Spodoptera</i> genus. Comparative genomic analyses of <i>Spodoptera</i> species that differ in host plant breadth identified a relatively conserved number of UGT genes in generalist species but high levels of UGT gene pseudogenization in the specialist <i>Spodoptera picta</i>. CRISPR-Cas9 knockouts of the three main UGT gene clusters of <i>Spodoptera frugiperda</i> revealed that UGT33 genes play an important role in allowing this species to utilize the poaceous plants maize, wheat, and rice, while UGT40 genes facilitate utilization of cotton. Further functional analyses in vivo and in vitro identified the UGT SfUGT33F32 as the key mechanism that allows generalist <i>S. frugiperda</i> to detoxify the benzoxazinoid DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), a potent insecticidal phytotoxin produced by poaceous plants. However, while this detoxification capacity is conserved in several generalist <i>Spodoptera</i> species, <i>Spodoptera picta</i>, which specializes on <i>Crinum</i> plants, is unable to detoxify DIMBOA due to a nonfunctionalizing mutation in <i>SpUGT33F34</i>. Collectively, these findings provide insight into the role of insect UGTs in host plant adaptation, the mechanistic basis of evolutionary transitions between generalism and specialism and offer molecular targets for controlling a group of notorious insect pests.