Mexenone protects mice from LPS-induced sepsis by EC barrier stabilization.

Choi, Yoon Ji; An, Jimin; Kim, Ji Hye; Lee, Sa Bin; Lee, Bo Seok; Eom, Chae Young; Lee, Hyohi; Kwon, Nayeong et al. · PLoS One · 2024

basic_science · Level V

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Abstract

Blood vessels permit the selective passage of molecules and immune cells between tissues and circulation. Uncontrolled inflammatory responses from an infection can increase vascular permeability and edema, which can occasionally lead to fatal organ failure. We identified mexenone as a vascular permeability blocker by testing 2,910 compounds in the Clinically Applied Compound Library using the lipopolysaccharide (LPS)-induced vascular permeability assay. Mexenone suppressed the LPS-induced downregulation of junctional proteins and phosphorylation of VE-cadherin in Bovine Aortic Endothelial Cells (BAECs). The injection of mexenone 1 hr before LPS administration completely blocked LPS-induced lung vascular permeability and acute lung injury in mice after 18hr. Our results suggest that mexenone-induced endothelial cell (EC) barrier stabilization could be effective in treating sepsis patients.

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